Gene Expression Profiling of Pseudomonas aeruginosa implicated in Otitis Media, Ile-Ife, South western, Nigeria

Otitis media is an inflammatory disease of the mucosal lining of the middle ear, which includes a variety of medical conditions with different signs and symptoms. It has a worldwide distribution, particularly among children.

A total of 170 patients between the ages 0-75 years who presented with sign and symptoms of otitis media at Obafemi Awolowo University Teaching Hospitals Ear, Nose and Throat clinic were enrolled for this study. Middle ear discharge were collected, and cultured on MacConkey agar, Chocolate agar and Blood agar. It was incubated for 24 hours at 37oC and was observed for growth. The organisms were characterized and identified using conventional biochemical method. Disc susceptibility tests were performed on bacterial isolates using Gentamycin, ciprofloxacin, Augumentin, fusidic acid, rifampicin, clindamycin, Chloramphenicol, erythromycin and tetracycline. It was incubated for 24hours and zone of inhibition was measured and compared to CLSI, 2016. Gentamycin Resistant gene were determined in multiple antibiotics resistant isolates by Polymerase Chain Reaction using ermA, ermC, and mefA primers.

Results indicated Pseudomonas spp (49%) has a high frequency of occurrence incriminated in otitis media followed by Streptococcus spp (22.5%), Staphylococcus spp (16%), Proteus spp (6%), Escherichia coli (2.5%), Klebsiella spp (2.5%) while Citrobacter spp (1.5%) has the least occurence. In the 0-5 and 6-11 year age groups in the two sexes, female patients in the two age groups were more infected than the male counterparts.

Pseudomonas isolates harboured strong resistance against Erythromycin and it showed three resistance gene ErmA. ErmC and MefA were present as revealed by Polymerase chain reaction.

Changes in the DNA sequence constitution of P. aeruginosa can induce various disorder including otitis media.

Author(s): Omololu-Aso J1* and Ogunyemi M

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