Evaluation of polyphenolic contents and antioxidant activity of wildly collected Ganoderma lucidum from central Himalayan hills of India

Ganoderma lucidum, a unique woody and spongy mushroom, belonging to family Ganodermataceae is widely distributed throughout the world. It is a highly medicinal mushroom which is being consumed for its pharmaceutical value rather than as food. To evaluate the polyphenolic contents (flavanoids) of both methanolic and aqueous extracts, and evaluate its anti- oxidant activity against ABTS [2, 2' –azinobis (3-ethylenebenzothiazoline-6- sulphonate)] and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radicals. Phenolic contents were determined by using Ci0calteu method and aluminium clod method respectively using quercetin catechol as a standard. Extract containing predominant amount of phenolic was evaluated for its anti- oxidant activity against ABTS and DPPH free radicals. And its activity was compared with quercetin and ascorbic acid. In this study, G. lucidum’s methanloic extract was found to contain 9.245 ± 0.184 mg Catechol Eq/gm sample dry weight of total phenolics and 2.140 ± 0.132 mg Quercetin Eq/gm sample dry weight of flavanoids which was quite higher than its aqueous extract. It was also found to possess anti- oxidant activity with IC50 value of 1.162 ± 0.016 mg/ml against DPPH and 0.555 ± 0.009 mg/ml against ABTS free radicals. These findings demonstrate that G. lucidum has a potent anti- oxidant activity which can be used in formulation of many anti- oxidant products. It can be use as a source of phenolics, and can be regarded as a potent herb for various ailments caused by oxidant species.

Author(s): Anshumali Rawat, M. Mohsin, P. S. Negi, A. N Sah and S. Singh

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