Evaluation of inhibitory activity of Vitex nirgundo and Terminalia chebula by alpha amylase inhibiton assay in management of diabetes

The present study was aimed to determine anti-diabetic activity of Vitex nirgundo and Terminalia chebula by alpha amylase inhibition assay. Powder of the herbal plants was subjected to cold maceration successively by petroleum ether, chloroform, ethyl acetate, n-butanol and water for 72 hrs and the extracts were filtered. Extracts were concentrated and weighed to get respective extractive values. Phytochemical screening was done to determine constituents present in every extracts. TLC fingerprinting done to determine the maximum constituents present in extracts of different solvents. The enzyme inhibition was determined by alpha-amylase inhibition assay by UV spectrophotomter at 540 nm using standerd curve.

Author(s): Umang Devani, Nancy Pandita and Yusuf Kachwala

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