Evaluation of Antibacterial Activity of Pongamia pinnata linn on Pathogens of Clinical Isolates

Introduction:Plants are well known for the presence of antimicrobial compounds. Our study was to screen the antibacterial activity of the seed extracts of Pongamia pinnata Linn.
Material and Methods: Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens, Micrococcus luteus, Proteus vulgaris and Klebsiella pneumonia were isolated and cultured from clinical samples obtained from Department of Microbiology, R.L.Jalappa Hospital and Research Center, Tamaka Kolar. Good quality seeds were collected from local region of Kolar and authenticated by College of Horticulture science. Extracts were made with methanol (M) and ethanol (E) solvents. A fixed inhibitory concentration of 100μg/ml of seed extract was tested by using Agar well Diffusion method and the same compared with the antibiotic Ceftazidime at equal concentration.
Results: Methanol extracts of Pongamia pinnata L(PPM) showed higher antibacterial activity than ethanol extracts of Pongamia pinnata L(PPE).
Conclusion: Pongamia pinnata L has good bactericidal activity against the selected Hospitalized pathogens and the maximum activity evinced on Pseudomonas aeruginosa with zone of inhibition 20mm by methanol extract and 18.5mm on Pseudomonas aeruginosa in ethanol extract in comparison to Ceftazidime.

Author(s): Mary Shobha Rani, C D Dayanand, Jeevan Shetty Pradeep Kumar Vegi, and A V Moideen Kutty

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