Efficient callus induction protocol for Sorghum bicolor

Sorghum, a hardy cereal crop, is a basic staple food foe many rural communities. Its adaptability to more drought prone areas gains attention for cultivation of this crop for food security. Six genotypes of Sorghum were examined for efficient protocol design in tissue culture studies, Tissue cultures were initiated from immature inflorescence on a modified MS-medium containing combination of auxins and cytokinins. Studies were carried out on callus induction, regenerable callus formation, callus fresh, dry and total weights, volume for the development of an effective regeneration. Of all the six Sorghum genotypes IS 462, IS 2898, IS 3477, IS 3541, IS 5291, IS 27703 studied, genotype IS 3477 showed highest callus induction. Among all the genotypes the MS-medium containing 2, 4-D 1.5mg /L+ 0.5KN mg/L showed good results. The growth rates of total callus, callus fresh and dry weights were also highest for the genotype IS 3477. The total callus volume was highest (80mm) on 12th week for the genotype IS 3541.

Author(s): Dora SVVN, Sarada Mani N and Sharmila Polumahanthi*

Abstract | PDF

Share This Article