Effects of Profenofos and Chlorpyrifos Pesticides on Oxidative Stress Biomarker, Biochemical Toxicity, DNA and RNA Content of Male Albino Mice

Objective: The present study aimed to evaluate the profenofos and Chlorpyrifos pesticides on oxidative stress biomarker, biochemical toxicity, DNA and RNA content of male Albino mice. Method: The animals were divided into three groups of equal number, The control group (1) was orally and daily administered with an equivalent amount of the vehicle (distilled water) for two weeks, the second group was given drinking water with 1.5 mg/kg BW of profenofos during two weeks of oral and daily administration and the third group were orally and daily administered with Chlorpyrifos (1.5 mg /kg BW). At the ends of the experimental period (2 weeks), the mice were sacrificed under diethyl ether anesthesia at fasting state. Results: The present results showed that the activities of Superoxide dismutase (SOD), Catalase and glutathione reductase (GR), enzymes in homogenate of snail’s tissues were decreased in the tissues of mice treated profenofos and Chlorpyrifos while lipid peroxide (LP) activity increased. Also, the profenofos and Chlorpyrifos pesticide caused a gradual significant reduction of enzyme activities of ALT, AST and ALP and increases in the glucose level and acid phosphatase in serum of male mice Also, the activity of glycolytic (PK, PFK and GPI) and gluconeogenic enzyme activities (F-1, 6-D-Pase) were significantly increased in tissues of treated mice. The change in the intensity number and position of DNA bands resulting from the profenofos and Chlorpyrifos pesticides can be attributed to the fact that herbicides can occur genotoxicity. Conclusion: The pollution the water environment by profenofos and pesticide chlorpyrifos, would negatively impact on the metabolism of mice and genetic toxicity products at low concentration and treatment for a long time and demonstrated the risk addicting herbicides to human health.

Author(s): Haleema Al-Nahari

Abstract | PDF

Share This Article