Effects of Adansonia Digitata Pulp Extract on Ovarian Damage Induced by Carbon Tetrachloride (CCl4) in Adult Female Wistar Rats (Rattus Novergicus)

Infertility, a couple’s inability to conceive after one year of unprotected regular intercourse, is an important issue in the world. The use of natural products in the treatment of infertility has been considered as a possible alternative to conventional therapies. This study was carried out in order to determine the curative effect of Adansonia digitata pulp extract on ovarian damage induced by carbon tetrachloride on the histology, level of hormone and the level of oxidative stress in the ovary of adult female Wister rats. Twenty adult female Wister rats were used as subjects for the experiment. The rats were then grouped into four (A, B, C, and D groups) containing five rats each. Group A (control) were given animal feed and distilled water, Group B rats were treated with 500 mg/kg of Adansonia digitata pulp extract, Group C rats received 2.5 ml/kg of carbon tetrachloride (CCl4 ), and Group D rats were treated with both Adansonia and CCl4 . Their ovaries were then removed and processed for histological examination, while their sera were taken for biochemical analysis and the supernatant from the homogenized tissues were used to analyse the level of oxidative stress. There were observable improvement in the structural appearance of the ovary of rats treated with Adansonia digitata pulp extract (Group B) while the ovary of rats treated with CCl4 (Group C) showed extreme damage to the structural outlook of the tissue. The ovaries of rats treated with both CCl4 and Adansonia digitata pulp extract showed an improvement in the damaged areas of the tissue. There was also an increase in the level of progesterone in rats treated with CCl4 compared to the control (Group A) and also an increase was observed in the level of eostrogen in the rats treated with CCl4 compared to control (Group A). The level of oxidative stress in the rats treated with CCl4 also increased when compared with the control group which indicates that damage has occurred to the tissue.

Author(s): Ogunbiyi OE , Oyewopo AO , Johnson Olawumi F, Adunmo GO and Oyabambi AO

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