Effect of temperature and changes in medium pH on enzymatic hydrolysis of β(1-4) glycosidic bond in orange mesocarp

This study evaluates Orange mesocarp as a feed stock for production of glucose syrup using Trichoderma. reesei. The material was crushed to 100 – 150 μm, 200 – 250 μm and 300 – 425μm particle sizes and fed into a bioreactor where delignification was effected.Enzymatic hydrolysis of the substrates was carried out at different temperatures, 30oC, 37oC, 40oC, 50oC and 55oC and the glucose produced at different temperature was analysed using Randox glucose kit and colorimeter. Medium of different pH was used for the enzymatic hydrolysis of orange mesocarp within the range of 3.0 – 6.0 using sodium acetate buffer. The optimal pH for the hydrolysis was evaluated by analysing glucose concentration produced at different pH level (Wen et al., 2004).Glucose concentration of 2.1mmol L-1, 2.3mmol L-1, 1.7mmol L-1, 2.3mmol L-1 and 2.3mmol L-1 were obtained as the maximum glucose concentrations at temperatures 30oC, 37oC, 40oC, 50oC and 55oC respectively. This indicates that the optimum temperature is 37oC and denaturation begins at a reaction time of 20 hours at temperature of 55oC. Optimum pH of 4.5 was observed for best enzyme activity.

Author(s): Ocholi Y, OduboGF and Yabefa JA*

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