Effect of substrate concentration and cell loading on the hydrolysis of β(1-4) glycosidic bond in orange mesocarp (Citrus sinensis) by trichoderma reesei for glucose production

In this study, we evaluate Orange mesocarp as a feed stock for production of glucose syrup using Trichoderma. reesei. The material was crushed to 100 – 150 μm, 200 – 250 μm and 300 – 425μm particle sizes and fed into a bioreactor where delignification was effected.A series of enzymatic hydrolysis was carried out at different substrate concentrations of 0.2g/L, 0.4g/L, 0.6g/L, 0.8g/L, 1.0g/L, 1.2g/L1.4g/L, 1.6g/L, 1.8g/L and 2.0g/L, while other reaction conditions were kept constant. Additional series of enzymatic hydrolysis was carried out at different cell concentration of 0.01gL-1, 0.02gL-1, 0.03gL-1, 0.04gL-1, 0.05gL-1, 0.06gL-1, 0.07gL-1, 0.08gL-1, 0.09gL-1 and 0.10gL-1. In separate runs, the effect of substrate concentration and cell loading on extent of hydrolysis was studied. Our results reveal that as the substrate concentration increases from 0.2gl-1 to 2.0gl-1, there was a corresponding increase in glucose concentration from 1.2mmolL-1 to 2.13mmolL-1 and 0.4mmolL-1 to 2.13mmol-1 at Cs = 0.6g and 1.2g respectively as time increases. Similarly, there was a substantial increase in glucose concentration with increase in cell loading.

Author(s): Ocholi Y, Odubo GF and Yabefa JA*

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