Effect of Phytohomones and chemical elicitors on growth and phenolic acids production in Solannum tuberosum cultivars through in vitro cultures, RP HPLC profiling of phenols and their antioxidant & anticancer potential

A current research work comprises effects of phytohormones and chemical elicitors on the multiplication of Solanum tuberosum shoots along with production of therapeutic compounds such as phenolic acids. Quantification assays of major antioxidants such as TPC, Catalase, SOD and GSH were done along with evaluation of antioxidant activity by adapting DPPH and ABTS methods from Solanum tuberosum. L cultivar regenerated in MS medium supplemented with different concentrations of Phytohormones and chemical elicitors respectively. Furthermore, in vitro regenerated plants were analyzed by reversed phase HPLC for the identification and quantification of major phenolic acids that showed the presence of Phenolics such as Quercetin, gallic acid, ferulic acid, sinapic acid, vanillic acid 4-hydroxy-3-methoxy benzoic acid, m-coumaric acid, trans-4-hydroxy 3-methoxy, cinnamic acid caffeic acid and syringic acid.

Author(s): Asif Saleem

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