E-BABE- graphene hybrids polymer composite nano-materials based on electrochemical DNA sensors

Electrochemical genosensors for the ultra-trace determination of label-free DNA hybridization with ultrahigh sensitivity, low cost, specifific selectivity and fast analysis can be developed for cancer diagnosis and food science in complicated samples using a variety of gold (Au) nanomaterials and their three-dimensional (3D) nanoarchitectures as well as layerby-layer (LBL) assembly. We explored the LBL assembled gold nanoparticles (AuNPs)/lower-generation (Gn≤3) polyamidoamine dendrimer (PD) with the reduced graphene oxide (rGO) core as electrochemical gene nanobiosensing platforms with 3D fractal nanoarchitecture for fast, ultra-trace determination of label-free DNA hybridization. Their morphologies, structures, electrochemical properties, and gene nanobiosensing performances were characterized. AuNPs/GG2PD displayed the best excellent structural stability, lowest mobility on solid surface with the increasing charge resistance, widest linear range (1.1 × 10−6 – 1 × 10−18), and the lowest limit of detection (1.87 × 10−19 M) in comparison with both AuNPs/GG1PD-based and AuNPs/GG3PD-based probes. This work will provide a new candidate for the development of metal nanoparticles functionalized PD with inorganic nonmetallic nanomaterials as cores with 3D fractal nanoarchitecture and promising electrochemical gene nanobiosensing platforms based on dendrimernanoinorganic hybrids with 3D nanoarchitectures and LBL assembly for fast and ultra-trace detection of lab -free DNA hybridization with potential application in bioanalysis and medical diagnosis of genetic diseases.

Author(s): Kumarasamy Jayakumar

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