Different Cell Culture Mediums Show Various Effects on CD8 + T Cells Expansion - A Bioinformatics Study

Expansion of T cells, especially CD8 ⁺ cells, is very important for cell therapy approached in diseases related to the immune system. Finding signaling pathways and molecules involved in improving the quality and quantity of T cells can be a great help in compensating the lost lymphocytes in the body. In this study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B  in 4-1BBL , Akt inhibitor, interleukin 7 and 15 expansion media.

Author(s):   Arsalan Jalili

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