Development of hptlc fingerprint by simultaneous estimation of ashwagandha
(withaferin-A) and glycyrrhiza(18-ÃÆÃ Â½Ãâò-glycyrrhetinic acid) in some polyherbal
over the counter marketed formulations
A new simple, precise, rapid and selective higḫ̢Ãâââ¬ÃâÃÂperformance thin̢̮Ãâââ¬ÃâÃÂlayer chromatographic (HPTLC) method has been
developed and validated for the simultaneous determination of withaferin A and 18-ÃÆÃ Â½Ãâò-Glycyrrhetinic acid in
Ayurvedic formulations containing Ashwagandha and Glycyrrhiza. The retention factors of withaferin A and 18-ÃÆÃ Â½Ãâò-
Glycyrrhetinic acid were 0.27 ̢̮â¬Å¡Ãâñ 0.02 and 0.72 ̢̮â¬Å¡Ãâñ 0.01 respectively. Linearity was obtained in the range of 1̢̮Ãâââ¬ÃâÃÂ5 ÃÆÃ Â½Ãâüg
mL̢̮Ãâââ¬ÃâÃÂ1 for withaferin A and 18-ÃÆÃ Â½Ãâò-Glycyrrhetinic acid. The developed and validated HPTLC method was employed for
standardization of three Ayurvedic formulations for their content of the two markers. Satisfactory recoveries of
98.39 to 99.77% 98.46 to 99.60% were obtained for withaferin A and 18-ÃÆÃ Â½Ãâò-Glycyrrhetinic acid. The results obtained
in validation assays indicate the accuracy and reliability of the developed simultaneous HPTLC method for the
quantification of withaferin A and 18-ÃÆÃ Â½Ãâò-Glycyrrhetinic acid in all three Ayurvedic formulations containing
Ashwagandha and Glycyrrhiza. Author(s): Trivedi Manisha N. and Santani D. D. Abstract |
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