Determination of the total content of diterpene glycosides in Stevia rebaudiana plant by the method of direct potentiometry

We have investigated the reaction of diterpene steviol glycosides with heteropolyanion of Keggin structure of 12- molybdophosphoric acid by UV and IR spectroscopy. The associative nature of the interaction was confirmed. The resulting slightly soluble associate was used as electrode active substance in plasticized membranes of ion-selective electrodes sensitive to steviol glycosides. The developed new film ion-selective electrodes, being sensitive to stevioside, have satisfactory electrode characteristics. They are proposed for the quantitative determination of steviol glycosides in extracts, food products and cosmetics by direct potentiometry. A method for the quantitative determination of diterpene steviol glycosides in extracts and industrial output by direct potentiometry has been developed. The developed method meets all the requirements of modern analysis - it is simple, express, safe and affordable. It has sufficient selectivity, accuracy, and sensitivity.

Author(s): Natalia V. Lutsenko, Maria O. Mironyak, Oksana V. Labyak, Olena V. Volnyanska and Volodymyr I. Tkach

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