Comparıson of amıno acıds for theır effıcıency on regeneratıon ın wheat embryo culture

Regeneration ability and regenerant length of Triticum durum Desf. “Ç-1252” and “Kunduru 1149” and Triticum aestivum L. “Bolal” and “Ä°kizce 96” cultivars investigated in this study. Mature embryos using as an explant source for callus production. Embryo derived calli from four wheat cultivars were cultured with MS + serine, alanine, glutamine or arginine (0.5 mM) to test their efficiency of regeneration rate and regenerant length. After surface sterilization of seeds, mature embryos seperated from seeds and transferred to MS medium containing 2 mg/l 2,4-D and 1,5 mg/l. Cultures were incubated at 25°C in dark for callus production. At the end of three weeks calli were transferred to hormone free MS regeneration medium containing various amino acids. There were significant differences in percentage of callus induction and regeneration capacity on the different amino acid medium. Amino acid applications increased the regeneration rate (P<0.01) and regenerant length (P<0.05) with varying degrees in almost all genotypes. These results indicate that genotype and medium had significant effects on regeneration capacity of cultivars.

Author(s): Ragbet Ezgi Duran*, Yasemin Coskun and Tunhan Demırcı

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