Comparison of Antioxidant Potential of Ezenus Versus Marketed Herbal Products in India: an In-vitro Study

The present investigation describes the comparative evaluation of Ezenus: a polyherbal, sugar free candy with twelve commercial products with respect to antioxidant potential. Total phenolics, flavanoids and thiol contents were estimated and antioxidant potential of Ezenus and commercially available products claiming to be anti-stress and detoxifier, were evaluated. Ezenus showed highest polyphenol content of 27.34±1.95 mg Gallic Acid Equivalent/100g was significantly higher (59.35%) than MCF-12 and flavanoid content was 291.20±10.05 μg Quercetin Equivalent/100g which is 60.34% higher than that for MCF-5. Total thiol of Ezenus was 1.09±0.08 μM/ml and showed highest percentage difference of 67.89% against MCF-6. Ezenus was found to be superior in inhibiting lipid peroxidation and Melondialdehyde content detected for Ezenus was 6.04±0.15 μmols MDA/g) whereas MCF-11 showed highest MDA level of 36.60±2.08 μmols MDA/g. IC50 value of Ezenus was found to be 423±28 μg/ml which was 3.5 times lower than MCF-6. Ezenus showed greatest inhibition with IC50 of 730±35 μg/ml followed by others. Highest reducing power was exhibited by MCF-9 (IC50 414±52 μg/ml) followed by Ezenus (IC50 440±21 μg/ml). Ezenus, MCF-1, MCF-3, MCF-6, MCF-7 and MCF-10 showed almost similar IC50 of 1000 μg/ml followed by MCF-9 and others. Highest hydroxyl radical inhibition was shown by Ezenus while MCF-12 showed minimum that is 1.6 times lower. The patented formulation Ezenus depicted greatest superoxide anion scavenging activity while MCF-4 showed lowest activity in terms of IC50 which was 2.7 times lower than that of Ezenus. The antioxidant assays results suggest that the patented formulation Ezenus demonstrated significant and higher antioxidant potential as compared to the other commercial formulation available in Indian market under same category. The formulation is very useful in combating the oxidative stress and maintaining general well being-stress free life.

Author(s): Manu Chaudhary and Vijay Naithani

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