Comparative Study of Enzymatic Markers in Seminal Liquids of Normospermic and Azoospermic Samples at Pasteur Institute of Cote D’Ivoire (IPCI)

This study is initialized to assess the enzymatic activity link to the presence or absence of spermatozoa. In fact, thirty nine ejaculates from men aged 31 to 42 years were collected. 17 normospermia and 22 azoospermia were found. Seminal fluid was obtained by centrifugation of the samples and freeze at 4˚C. Those tests revealed there is no significant difference in term of the age, the bit with the enzyme markers; the statistical analysis show significant variations in certain parameters. Thus, for the Aspartate aminotransferase (AST), we notice a difference between normospermia with the values of 224.17 ±17.30 UI/l and azoospermia with the values of 143.13 ± 13.69 UI/l. These remarks were made for the γ-Glutamyl Transferase (GGT) with 16938 ± 1795 UI/l corresponding to the normospermia which are different from the azoospermia with the following values of 11357± 1326 UI/l. The consideration of these parameters could be envisaged after an assessment on a broad sampling in order to confirm the effectiveness of the presence of the spermatozoa according to the activity of these enzyme markers.This study is initialized to assess the enzymatic activity link to the presence or absence of spermatozoa. In fact, thirty nine ejaculates from men aged 31 to 42 years were collected. 17 normospermia and 22 azoospermia were found. Seminal fluid was obtained by centrifugation of the samples and freeze at 4˚C. Those tests revealed there is no significant difference in term of the age, the bit with the enzyme markers; the statistical analysis show significant variations in certain parameters. Thus, for the Aspartate aminotransferase (AST), we notice a difference between normospermia with the values of 224.17 ±17.30 UI/l and azoospermia with the values of 143.13 ± 13.69 UI/l. These remarks were made for the γ-Glutamyl Transferase (GGT) with 16938 ± 1795 UI/l corresponding to the normospermia which are different from the azoospermia with the following values of 11357± 1326 UI/l. The consideration of these parameters could be envisaged after an assessment on a broad sampling in order to confirm the effectiveness of the presence of the spermatozoa according to the activity of these enzyme markers.

Author(s): COULIBALY Founzégué Amadou, N’ Zi Konan Sylvère, AYEKOUE Eric, COULIBALY Noël, YAYE Yapi Guillaume and DJAMAN Allico Joseph

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