Comparative studies on growth parameters and physio-chemical analysis of Pleurotus ostreatus and Pleurotus florida

The present investigation clearly indicated that the Potato Dextrose broth has produced more number of spores followed by maize grains. The results of the study carried out on carbon requirement of P.ostreatus and P. florida showed that dextrose was the best carbon source closely followed by fructose, sucrose and galactose. Of all the tested nitrogen sources, the best mycelia yield was recorded in the medium that contained casein followed by urea, malt extract, peptone, yeast extract and ammonium chloride. Amino acids arginine stimulated the best yield followed by glutamic acid and cysteine. Of all the carbon/nitrogen ratios used ratio 5:3 was best for growth of Pleurotus ostreatus and Pleurotus florida. The growth was significantly enhanced in the medium suggests that all the macro-elements used are required for the growth of this fungus although the level of utilization varies. Magnesium is the best utilizable macro-element, followed by calcium, potassium and sodium. On Potato Dextrose medium the optimum temperature was 250C - 300C, the optimum pH was 5.5 and the best growth was observed in the inoculants of NaCl concentration 0.5% and 2.5%.

Author(s): Sridevi . Neelam, Srilakshmi. Chennupati and Sumer Singh

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