Comparative Effect of Different Nutrient Media on Regeneration Potential of Petiole Explants of Inula Royleana DC., A Multipurpose Plant Species of Kashmir Himalaya

During present study, effect of different nutrient media viz., MS, Nitsch, B5 and White media, was observed on petiole explants Inula royleana; in order to establish an efficient regeneration protocol for conservation of this one of the medicinally reputed plant species of Kashmir Himalaya. Among these nutrient media, callus induction and shoot regeneration were obtained only on MS and Nitsch media while as White medium proved effective in regenerating roots. MS proved more effective than Nitsch medium in inducing both maximum amount of callus as well as maximum mean number of shoots (9.5 with 4.7 cm mean shoot length) but number of days taken were more in comparison to Nitsch medium. When MS was supplemented with BAP (0.8 mg/l), maximum amount of callus was produced within 35 days in 40% cultures. However, for regenerating maximum shoots from this callus MS required BAP in combination with IAA at concentrations 5 mg/l and 2 mg/l respectively. Regenerated shoots produced roots on White basal medium and complete plantlets were then acclimatized with 70% survival rate by using vermicompost and soil in 3:1 ratio.

Author(s): Samar Amin*

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