Chromatographic profiling using RP-HPLC and Estimation of Biochemical Parameters of Saussera lappa

The current phytochemical investigation deals with the chromatographic fingerprinting of the methanolic extract using High Pressure Liquid Chromatography using a RP C-13 column and a Photo Diode Array Detector of the dried roots of S.lappa for the detection of the chemical constituents in the plants. Efforts were also made for the quantitative determination of the biochemical parameters for studying the biological efficacy of the plant. The current study revealed that the plant is very much rich in Phenolics and the results also denote that the plant possess a significant F/P ratio which is very near to the standard value one which implies the biological potency of the species. The HPLC profile enabled the separation of 19 constituents from the methanolic extract.

Author(s): Thara K. M* and K. F. Zuhra

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