Chromatographic Determination of Allelochemicals (Phenolic Acids in Jatropha curcas by HPTLC

Free and esterfied phenolic acids of Jatropha curcas were extracted with Methanol in soxhlet apparatus and determined by HPTLC. The phenolic acid such as kaempferol, coumarin, catechin, and quercetin acids were detected by the Linomat 5 (Camag, Switzerland). The Linomat 5 is controlled by wincats planar chromatography manager, version 1.3.4. Plate dimensions, number and distance of tracks, names of samples and volumes to be applied on to each track are conveniently programmed and saved as wincats analysis file. The planar chromatogram was developed with Toluene-Acetone-Formic acid (4.5: 4.5: 1) and the developed plate were sprayed with respective spray reagent and dried at 100°C in Hot air oven. The plate was photo-documented at Day light and UV366nm using Photo-documentation (Camag reprostar 3) chamber.

Author(s): S. Rejila, N. Vijayakumar and M. Jayakumar

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