Characterization of Oryza sativa Root Germin-like Protein Gene 1 Promoter in a Model Plant

TGermins and Germin-like proteins (GLPs) play important
role against various stresses. Our research group is
working on the characterization of Germin and Gemrin-
like Proteins. Presently, promoter region of Oryza
sativa Root Germin-like Protein 1 (OsRGLP1) gene was
fused with GUS reporter gene and designed expression
construct was used for plant (Nicotiana tabacum)
transformation. Transgenic plants were analyzed under
wounding, salt, drought and ABA by RT-PCR. In wounding
stress a gradual increase in GUS activity with time
was observed. In RT-PCR highest activity in leaves was
seen after 5 h with 4 fold increase in GUS transcript level
as compared to control. In salt stress transgenic plants,
highest activity was noted after 24 h at 250 mM showing
3-fold increase in GUS activity. In drought stress transgenic
plants were grown on different concentrations of
PEG solutions and GUS activity was noted after 24 h, in
which highest expression was observed at PEG 30 % both
visually and in RT-PCR data by showing 25-fold change
in GUS transcript level. Similarly, in ABA stress transgenic
plants were treated with a range of different concentrations
of ABA and the GUS activity was observed after
24 and 48 h in which highest activity was noted after 48
h at 450 µM which was 28-fold change. Moreover, histochemical
analysis showed strong GUS activity in different
parts of the transgenic plants. Induction of this promoter
showed its ability of driving gene expression under these
stresses, which carries particular importance in the production
of agronomically important transgenic crops..

Author(s): Tariq Mahmood

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