Biomimetic biocompatible hydrogel composed of jelly fig polysaccharide and lauric acid accelerates wound tissue regeneration: An in vitro study

Tissue regeneration is an essential process to maintain a normal
anatomical structure and function of the skin. Natural biopolymer-
based polymeric hydrogels are capable of absorbing a
large quantity of water to maintain the moisture environment
at the desired site. The eminence of hydrogels should be biocompatible,
nontoxic, keep the moist environment to supply
oxygen and nutrients, protect the wound against microbes and
absorb enough wound exudates. Here, we have prepared hydrogel
using jelly fig polysaccharide extracted from achenes of jelly
fig (Ficus pumila var. awkeotsang) as base material and loaded
with lauric acid (0.5 and 1%) as a bioactive ingredient to promote
the tissue regeneration. The physiochemical properties of
the hydrogel such as swelling, degradation, morphology, chemical
nature (ATR-FTIR) were thoroughly characterized. Also,
the biocompatibility of the hydrogel was studied using NIH
3T3 fibroblasts by MTT assay, live/dead and cell proliferation
by DAPI/FDA staining. Further, tissue regeneration ability was
assessed by in vitro scratch wound assay. MTT assay showed
that jelly fig (JF) hydrogel and lauric acid loaded jelly fig (JF +
LA) hydrogels did not show any toxicity. DAPI staining showed
more live cells on hydrogels compared to control (Tissue culture
plate). Cell proliferation was increased in (JF + LA 1%)
hydrogels compared to control. In vitro scratch wound assay
revealed that (JF + LA 1%) hydrogels improved the cell migration
and completed wound closure within 24 h. Hence, the JF
+ LA hydrogels can be used as an ideal wound dressing for the
acceleration of wound repair and tissue regeneration.

Author(s): Thangavel Ponrasu

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