Biodegradation of Almond Oil Driven Bovine Serum Albumin Nanoparticles for Controlled Release of Encapsulated Pearl millet Amylase

Amylase plays vital role in food, textile, leather, pharmaceutical and detergent industries due to its efficacy and industrial viability. In our work, amylase was extracted from Pearl millet (Pennisetum glaucum) and encapsulated in bovine serum albumin nanoparticles by chemical modification with almond oil, n-butanol and glutaraldehyde. Hence, bovine serum albumin was found to be favourable matrix for encapsulating extracted amylase due to its well known exploitable properties e.g. biocompatibility, non-antigenicity and non-toxicity. Biodegradation of almond oil driven enzyme loaded bovine serum albumin nanoparticles was performed by using different units of alkaline protease (10U, 15U, 20U, 25U, 30U, 35U, 40U, 45U & 50U) to study controlled release of encapsulated amylase. Kinetic parameters were also studied for Pearl millet extracted amylase and encapsulated amylase for their comparative analysis for optimal pH, incubation time, substrate concentration, CaCl₂ concentration and temperature for maximal enzyme activity which was tested by dinitrosalicylic acid method. Thermal stability at 70ºC was found to be 3 hours 30 minutes of encapsulated amylase and 50 minutes only for free amylase. Storage stability at 4ºC was observed 12 months for encapsulated amylase and one day only for free enzyme. Characterization of prepared bovine serum albumin was done by Dynamic Light scattering (DLS) and Scanning Electron Microscopy (SEM).

Author(s): Kirti Rani, Chandrika Gupta and Chanchal Chauhan

Abstract | PDF

Share This Article