Biochemical Profile of Enteromorpha linza
(L.) J.Ag. in hare Island, Thoothukudi,
Tamilnadu, India

John Peter Paul J.; Muthu Sheeba M.

Abstract

Biochemical Profile of Enteromorpha linza (L.) J.Ag. in hare Island, Thoothukudi, Tamilnadu, India

The aim of the study was to investigate the biochemical profile of Enteromorpha linza (L.) J. Ag. in Hare island, Thoothukudi, Tamil Nadu, India. The biochemical composition such as total carbohydrates, total proteins, total lipids, total phenols, total chlorophylls and total carotenoids were estimated using the standard procedure in fresh plant material. The preliminary phytochemical analysis was performed by Harborne method. The phytochemical screening of methanol extract was estimated using the standard procedure for UV-Vis spectroscopic, HPLC and FTIR. In the present study it was found that Enteromorpha linza (L.) J. Ag. contained high level of carbohydrates (33.8% FW), followed by proteins (20.8% FW), lipids (1.74% FW), phenols (1.65% FW), chlorophylls (0.038%) and low level of carotenoids (0.021%). The UV-Vis phytochemical profile of methanol extract of Enteromorpha linza (L.) J. Ag. was analyzed at 240 to 800nm. The results of FTIR analysis showed different peaks at 495.67, 538.10, 609.46, 651.69, 727.11, 732.18, 827.41, 939.27, 993.27, 1116.71, 1191.93, 1388.65, 1463.87, 1622.02, 2264.27, 2503.43, 2640.37, 2678.94, 2742.59, 2850.59, 2920.03, 2954.74, 3014.53, 3321.19, 3379.05 and 3402.20. It was confirmed the presence of functional groups such as amides, phosphorus compound, alcohols, phenols and halogen compounds. The qualitative HPLC fingerprint profile of methanol extract of Enteromorpha linza (L.) J. Ag. was selected at a wavelength of 254 nm due to sharpness of the peaks and proper baseline. The profile displayed two prominent peaks at the retention time of 1.417min and 2.190min followed by four moderate peaks were observed at the retention time of 0.215min, 0.298min 0.395min and 1.417min respectively. The biochemical analysis of Enteromorpha linza (L.) J. Ag. showed the presence of various phytochemicals. The results of the present study supplement the usage of the studied plant which possesses several bioactive compounds and used as food and medicine.