Application of zinc oxide nanoparticles loaded with slug slime as surgical glue for wound closure and healing

Zinc Oxide (ZnO) nanoparticles are already proven as
the most effective adhesive having a good zeta potential,
adhesive property, microbial activity and flow resistance
among all the nanoparticles tested for bioadhesion. Interestingly,
defensive mucus secreted by slug tested to fall in
amine group is strong, sticky, highly flexible, capable of
withstanding the everyday stress, the tissue in the body
has to bear. A new approach has been adopted for rapid
closing and healing of the wound using surgical glue prepared
by loading ZnO nanoparticles into slug slime. ZnO
nanoparticles were prepared by direct precipitation method
without capping agent and characterized by Fourier
Transform Infrared Spectroscopy (FTIR) analysis whereas
X-Ray Diffraction (XRD) was done to calculate the crystalline
size of ZnO nanoparticles. The surgical glue prepared
by loading nanoparticles with slug slime acted as
an effective adhesive for wound closure and healing. The
slime, ZnO nanoparticles and the prepared surgical glue
underwent the cytotoxicity test in HeLa cells confirming
that the viability of the cells is high in the group treated
with glue. In vivo testing included n-butyl cyanoacrylate
and suture as positive control for the glue which was
demonstrated for adhering incised epidermal, dermal
and sub-cutaneous layer of skin tissues of 6 mice in each
group of design. Surgical glue resulted in rapid healing
and adhesion providing good integration between the tissues
benefited over the cellular trauma caused by suture.
The histological analysis hinted the increased angiogenesis
and anti-inflammatory activity in the study groups
treated with surgical glue.

Author(s): Chanda Bhandari, Sailesti Joshi, Rameshwar Adhikari, Sulav Bastola

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