Antioxidant Effect of Curcumin Iron Complex of U87 Cells Induced by Beta-Amyloid

Curcumin iron complex could be utilized effectively for the treatment of Alzheimer's disease and anticipated that it could be evaluated among alternative treatment strategies in the field of medicine. The present study was conducted to investigate the antioxidant activities of iron with curcumin complex. In the study; three groups were formed as the control group, the Aβ group, and the Aβ⁺ curcumin iron complex group. Firstly, the cytotoxic potential of curcumin iron complex in U87 cells was investigated by the colorimetric MTT (3-4, 5-dimethylthiazolyl- 2,5 diphenyltetrazolium bromide) test. To determine the antioxidant status in the cell line treated with curcumin iron complex, examine the effects of superoxide dismutase (SOD) and catalase (CAT) activities were measured by ELISA method. The SOD enzyme level increased significantly in the curcumin iron complex group after curcumin was applied to the U87 cell (p<0.05). The results herein illustrated that CAT enzyme level (pg/ml) decreased after curcumin iron complex administration in all the groups except for the control group (p<0.05). When compared to the control group, the SOD and CAT levels were increased in the U87 cell line exposed to curcumin. Curcumin iron complex has a protective effect by increasing antioxidant parameters in the U87 cell lines.

Author(s): GuluzarOzlobat and Ares Alizade

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