Anti-oxidant, Anti-microbial Evaluation and GC-MS Analysis of Cyperus rotundus L. Rhizomes Chloroform Fraction

Introduction: Plant has been used for the treatment of diseases all over the world and is known to contain substances that can be used for therapeutic purposes or as precursors for the synthesis of useful drugs.
Objective: To analyses chloroform extract of Cyperus rotundus rhizomes using GC-MS and to evaluate anti-oxidant, anti-microbial activities.
Method: The dried rhizomes of C. rotundus extracted with 90% methanol (MeOH) (4 X 500 ml) under reflux and has subjected to column chromatography. The anti-oxidant activity of C. rotundus rhizomes extracts in solvents of varying polarity has measured radical scavenging ability, using the stable radical, DPPH. The different extracts of C. rotundus rhizomes has evaluated for anti-microbial activity against clinical isolates of Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Aspergillus niger, Aspergillus flavus and Candida albicans has selected based on anti-microbial activity of available drugs. Result: GC-MS analysis led to identification of five compounds namely (1) Trans-(2-chlorovinyl) dimethylethoxysilane, (2) 5-hydroxymethyl furfural, (3) Vanillin lactoside, (4) 2-propenoic acid, 3-(4-hydroxy-3-methoxy phenyl)-methyl ester, (5) 9, 12, 15-octadeca trienoic acid, 2, 3-bis [(trimethyl) oxy] propyl ester is the first report in the chloroform extracts of Cyperus rotundus rhizome.
Conclusion: The results showed the CHCl3 fraction of C. rotundus rhizomes can be considered as good sources of natural antioxidants and antimicrobial compounds present in it and has incorporated into the drug theories.

Author(s): Aeganathan R., Rayar A., Ilayaraja S., Prabakaran K., Manivannan R.*

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