Antinociceptive activity of methanol extract of Hyoscyamus reticulatus L. in mice

In the present study acute toxicity and possible antinociceptive effect of the metanolic extract of Hyoscyamus reticulatus L. was investigated in Swiss - albino mice of either sex. To assess Hyoscyamus reticulatus L. extract’s acute toxicity it was given intraperitonelly to the mice (6 in each group) in dose 50, 100, 200, 400, 800, 1200 and 1600 mg/kg body weight. After probit analyze of mortality at 24, 48 and 72h after administration, 25, 50 and 100 mg/kg were determinate as safe doses for antinociceptive evaluation. Two models were used to study the effects of the extracts on nociception, acetic acid-induced writhing test and hot plate test in mice. Hyoscyamus reticulatus L. extract was administered intraperitoneally 30 minutes prior to pain induction. The metanolic extract in 50 mg/kg dose showed significant (p<0.05) analgesic activity comparable with diclophenac sodium, evidenced by increase in the reaction time by hot plate method and significant (p<0.05) reduction in acetic acid - induced writhings in mice in 100mg/kg dose with a maximum effect of 35.56 % reduction. These effects were compared with the control and standard drug, diclophenac sodium (50 mg/kg, p.o). The results indicate that metanolic extract of Hyoscyamus reticulatus L. possesses a significant antinociceptive activity in both central and peripheral pain models in mice and therefore, it can be used as supplemental therapy in acute or chronic pain conditions.

Author(s): Oto G, Ozdemir H, Yaren B, Yetkin Y, Tas A, Tanrıtanır P, Öztürk F

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