Antigenic relationships of citrus yellow mosaic virus by immunological methods

Citrus yellow mosaic virus infecting citrus species like rangapur lime, acid lime and sweet orange was detected by different immunoassays like Agarose double diffusion test (AGDDT) and Dot Blot ELISA by using homologous polyclonal antiserum. The Citrus yellow mosaic virus infecting rangapur lime, acid lime and sweet orange was reacted positively with homologous antiserum raised against rangapur strain in AGDDT and Dot Blot ELISA. In agarose double diffusion test a precipitin line was observed with homologous antiserum and with purified virus. Positive reaction was also observed with homologous antiserum and purified virus samples of rangpur lime, sweet orange and acid lime and partially purified samples of Canna indica, maize and sorghum. The CYMV infected rangpur lime, sweet orange, acid lime, Canna indica, maize and sorghum crude leaf samples reacted positively with homologous antiserum in Dot-blot ELISA.

Author(s): Susmila Aparna Gaddam*, Venkata Subbaiah Kotakadi, M. N. Reddy and D. V. R. Saigopal

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