Antibacterial, antioxidant and antiglycation potential of Costus pictus from Southern region, India

Methanol and aqueous extracts of Costus pictus was tested in vitro for their antibacterial and antioxidant activities. An antibacterial activity analysis was carried out using agar well diffusion and broth dilution techniques. The antibacterial assay of leaf, root, stem and flower extracts of C. pictus against four bacteria are presented. The minimal inhibitory concentrations (MICs) of all the extracts of C. pictus were found to be between 20-30μg/ml. Antioxidant activity of the extracts was determined by DPPH radical scavenging capacity assay. Methanol extracts showed the highest antioxidant activity. The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteau reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The total phenolic content ranged from 148.67 ± 0.83 to 233.83 ± 0.44 μg of gallic acid/g of plant extracts. The flavonoid content varies from 370.08±0.96μg of quercetin/ g of plant extracts. The reduction of NBT by Amadori products was inhibited by incubating glucose/ BSA system with different parts of C. pictus (leaf, root,stem and flower) and aminoguanidine, and every parts showed a stronger inhibitory effect than aminoguanidine . The ability of the test samples to inhibit NBT reduction was in the order of stem methanol >flower methanol > root methanol> leaf methanol. The present study reveals that the selected plant would exert several beneficial effects by virtue of its antioxidant and antiglycation activity and could be harnessed for drug formulation.

Author(s): Mala Majumdar and Prachi S. Parihar

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