Antibacterial and Antioxidant Activities of Aegle marmelos and Cassia fistula Leave Extract

In this study, we investigated antibacterial and antioxidant activities of ethyl acetate and methanol extracts obtained from leaves of Aegle marmelos and Cassia fistula. Bacteria used in this study were Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated based on the diameter of inhibition zone (in millimeters, mm) using disk diffusion method and well diffusion method. MIC values of the plant extracts against these bacteria were assessed using broth dilution method. Result shows that leave extracts of these plants have varying degrees of antibacterial activity on tested microorganisms. Plant leaf extract in methanol shows quite good antibacterial activity in compare to extract in ethyl acetate. Therefore methanol extract can be used as an antiseptic in infection caused by these bacteria. This is alternative treatment method when these bacteria have resistance against antibiotics. Next, we evaluated DPPH Radical scavenging activity, Nitric oxide radical scavenging activity and reducing power of the ethyl acetate extract prepared from leaves of Aegle marmelos and Cassia fistula. Plant leaf extract in ethyl acetate shows significant scavenging activity as DPPH, Reducing power and Nitric oxide scavenging in compare to reference. Reference standard used in this study was ascorbic acid, which also has scavenging activity in significantly increased manner. Result of qualitative analysis shows that plant leaf extract prepare in ethyl acetate has the high antioxidant activity. Different leaves extracts with strong antioxidant activity indicates its scope for utilization in food and biological systems.

Author(s): Kiran Abha Singh and Anup Kumar

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