Antibacterial Activity of Probiotics (Lactobacillus Spp.) against Intestinal Pathogenic Bacteria

This work aimed to determine the antibacterial activity of probiotic bacteria (Lactobacillus spp.) isolated from fermented milk products against pathogenic bacteria (Escheria coli, Salmonella typhi and Staphylococcus aureus). Lactobacillus bacterium was isolated from dairy products include fermented milk (Yoghurt). Samples collected from Khartoum state, they were cultured in MRS agar, incubated at 37°C for 72 h. Then they have been identified by biochemical tests. After that, Lactobacillus was cultured in MRS broth, incubated for 48-72 h. The antimicrobial compounds were extracted by centrifuge, then the filter paper was saturated with the extract. Three pathogenic isolates were imported. Then the antibacterial activity test was done, using agar diffusion test by placing the saturated filter paper on the culture of tested bacteria. It was observed that the gram negative bacterium (E. coli) was more sensitive than gram positive bacteria (Staphylococcus aureus).

Author(s): Amna Yousif Mohamed*, Ahmed Ali Mustafa, Asim Hassan Omer, Abdalrhman Abd Elkaerem and Mohamed Omer Taha

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