Anther Culture in Potato (Solanum tuberosum L.) in vitro

Double haploid (DH) plants Production is a valuable tool
in plant breeding programs. Since conventional breeding
takes long time for the development of newly improved
cultivars, this technique reduces the time needed for this
purpose. Practically, application of (DH) technology in potato
breeding through androgenesis in Santana cultivar is
possible by considering number of factors that influence
androgenesis. Culture medium has to be considered in
respect of constituents to fulfill the needs of culture target
where N6 medium significantly showed better results
(14.61%) if compared to MS medium (8.78%) anthers
produced embryos. Auxin/cytokinin represented in this
study by 2,4-D/BA showed better results in induction of
embryogenesis when combined together at (2.0 mg/l 2,4-
D and 0.5 mg/l BA) which resulted in (35.67%) anthers
produced embryos rather than each one of them alone.
Enhanced results were obtained after floral buds pretreatment
with thermal chock with three different degrees
(4°C, 25°C, 30°C) for two different exposure time (48
h and 72 h). N6 medium showed the highest percentage
of anthers produced embryos (44%) when supplemented
with 2 mg/l 2,4-D+0.5 mg/l BA, while pretreatment with
4°C for 72 h favored the embryos production (16.67%)
followed by (14.00%) and (12.00%) at 25°C and 4°C for
48 h respectively with no significant difference among
them. Pretreatment with 32°C for (48 h and 72 h) resulted
in (4.67%) and (5.33%) respectively differed significantly
form the low temperature. Silver nitrate has shown
to be potent at 2 mg/l in inhibiting ethylene, increasing
embryogenesis (60.0 %) and reducing the non-responsive
anthers (6.67(%.

Author(s): Aboshama HM and Atwa MM

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