ANKKI / Taq A1 Genotyping from Dried Blood Stored on Filter Paper Among Alcohol & Nicotine Dependant Subjects

This paper describes the use of blood spotted filter paper to study the genotyping of ANKKI / Taq A gene. For the study hundred patients visiting National Drug Dependence Treatment Centre (NDDTC) for the treatment of co-morbid alcohol and nicotine dependence were selected at random. Blood was spotted on to Whatman 903 filter paper, dried and stored at room temperature. Genomic DNA was extracted and genotyping was carried out from dried blood and corresponding whole blood samples. All the samples collected on to filter paper showed exact match of the genotyping when compared to fresh blood. At the end of 6 months of storage the genotyping was carried out successfully from all the samples. The present study shows that dried blood is suitable for ANKKI/ Taq A1 genotyping up to a period of 6 months at room temperatures.

Author(s): Rizwana Quraishi and Raka Jain

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