An acute oral toxicity study of methanolic extract from Tridex procumbens in Sprague Dawley’s Rats as per OECD guidelines 423.

Toxicology may be defined as the study of harmful, poisonous and adverse effects of drugs and other chemicals constituents found in plants, which may increase the chances of mortality or weakness in the general health, physically as well as mentally. The present study has been under taken to study the adverse or hazardous effects of methanolic extract from Tridex procumbens, dissolved in Dimethyl sulphoxide (DMSO) & accordingly to determine the LD50, to establish the safety of methanolic extract of Tridex procumbens in SD Rats as per OECD guidelines 423. All the Rats were sequentially administered orally the methanolic extract first in a single dosage of 2000 mg/kg body weight. All the animals were observed for mortality, wellness parameters and body weight for 14 days and due to some morbidity and mortality the experiment was again performed at same dosage and same results were observed. Then decrease in the dosage to 300 mg/kg body weight was performed and accordingly observed as per OECD Guidelines 423. No mortality or any significant change was observed at 300 mg/kg body weight, however at 2000 mg/kg body weight dose the mortality rate was 2/3. Conclusively indicates the LD50 value of Tridex procumbens methanolic extract to be less than 2000 mg/kg body weight and more than 300 mg/kg body weight (LD50 ≥ 300 mg/kg body weight, but < 2000 mg/kg body weight).

Author(s): Abrar Hussain Mir, Manjusha Sexena and Mohd Yousuf Malla

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