A study on comparative anti microbial and wound healing efficacy of solvent extracts and succulent leaf extract of Mikania scandens (L.) Willd.

Objective: A study for validation of reported wound healing property of fresh extract of succulent leaves of Mikania scandens (L.) Willd. was performed along with contemporary solvent extracts made from dry leaves of the plant, as the rural and ethnic people use the plant in this form.
Methods: The reported wound healing activity of the plant was studied in a novel way. For antimicrobial activity study, extract of succulent leaves was studied along with conventional methanolic and aqueous extracts of the dry leaves of the plant. The extracts were tested on Escherichia coli, Salmonella enteritidis and Staphylococcus aureus and the antimicrobial activity was tested both by conventional single disc diffusion method and a novel Spectrophotometric method. The wound healing and haemostatic activity were tested on induced punch wound of rabbit. Qualitative analysis for identification of phytochemicals present in the succulent leave extract was also performed.
Results: The succulent leave extract showed better antimicrobial activity against Salmonella enteritidis and Escherichia coli than methanolic extract, but showed lower activity against Staphylococcus aureus in disc diffusion and showed activity at 33% concentration against all the tested microorganisms in spectrophotometry. The methanolic extract of 5 mg/ml concentration failed to show any effect and the higher concentration of it as well as the fresh leave extract above 33% concentration could not be evaluated due to presence of color materials. The succulent leave extract showed better wound healing potential than methanolic extract on rabbit wound. It took 25 seconds for haemostasis in 8 mm punch wound of rabbit. The fresh extract contains Alkaloid, Carbohydrate, Glycoside, Saponin, Protein and Amino Acid and also Phenolic Compound.
Conclusion: It was found that the fresh extract of the succulent leaves of Mikania scandens (L.) Willd. plant is having better antimicrobial activity and wound healing potential than methanolic extract on rabbit wound. The fresh extract can be used as haemostatic agent also.

Author(s): Shibabrata Pattanayak, Partha Das, Tapan Kumar Mandal, Pratip kumar Debnath and Susanta Kumar Bandyopadhyay

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