A novel RP-HPLC method development and validation of Perindopril Erbumine in bulk drug and tablet dosage form

Some literatures revealed the high performance liquid chromatography (HPLC) method for perindopril erbumine. However, these methods are time consuming, so it is necessary to develop a cost-effective and less time consuming method for the estimation of perindopril erbumine in API as well as pharmaceutical formulation. The separation was performed on a symmetry C18 (4.6 x 100 mm, 5mm, Make: Phenomenex) column in an isocratic mode with the mobile phase consisting a mixture of Phosphate Buffer: methanol (30:70 v/v) was used as a mobile phase and the pH was adjusted to 5 by using O-phosphoric acid. The mobile phase was pumped at a flow rate of 0.8 mL min-1 and eluents were monitored at 215 nm. The selected chromatographic conditions were found to analyse perindopril erbumine (retention time = 2.45 min). The proposed HPLC method was validated with respect to linearity, accuracy, precision, robustness, ruggedness, limit of detection (LOD) and limit of quantification (LOQ). All the parameters were found to be within limits, which indicate that the above method was accurate and precise. Hence, it was concluded that the developed method is suitable for routine analysis of perindopril erbumine due to its less analysis time.

Author(s): Yasmeen

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